Advances in nanodisc platforms for membrane protein purification

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Drugging membrane protein interactions.

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Surface plasmon resonance spectroscopy for characterisation of membrane protein–ligand interactions and its potential for drug discovery.

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Structure determination of membrane proteins by NMR spectroscopy.

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Overcoming the challenges of membrane protein crystallography.

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Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers.

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Functional reconstitution of β2-adrenergic receptors utilizing self-assembling nanodisc technology.

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Directed self-assembly of monodisperse phospholipid bilayer nanodiscs with controlled size.

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Phospholipid phase transitions in homogeneous nanometer scale bilayer discs.

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Functional and structural comparison of the ABC exporter MsbA studied in detergent and reconstituted in nanodiscs.

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Probing membrane protein assembly into nanodiscs by in situ dynamic light scattering: A2a receptor as a case study.

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Real-time monitoring of binding events on a thermostabilized human A2A receptor embedded in a lipid bilayer by surface plasmon resonance.

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Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc.

Nature. 2020; 579: 297-302

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The structure of the polycystic kidney disease channel PKD2 in lipid nanodiscs.

Cell. 2016; 167: 763-773

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Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers.

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Structure of the human TRPM4 ion channel in a lipid nanodisc.

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Nanodiscs for immobilization of lipid bilayers and membrane receptors: kinetic analysis of cholera toxin binding to a glycolipid receptor.

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Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties.

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A saposin–lipoprotein nanoparticle system for membrane proteins.

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Saposin lipid nanoparticles: a highly versatile and modular tool for membrane protein research.

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The styrene–maleic acid copolymer: a versatile tool in membrane research.

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Polymer nanodiscs: advantages and limitations.

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Polymer nanodiscs: discoidal amphiphilic block copolymer membranes as a new platform for membrane proteins.

Sci. Rep. 2017; 7: 15227

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Lipid nanodiscs via ordered copolymers.

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Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum.

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Development of methodology to investigate the surface SMALPome of mammalian cells.

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Production of human A_2AAR in lipid nanodiscs for ¹⁹F-NMR and single-molecule fluorescence spectroscopy.

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Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR.

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DirectMX – One-step reconstitution of membrane proteins from crude cell membranes into salipro nanoparticles.

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Saposin–lipoprotein scaffolds for structure determination of membrane transporters.

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Influence of DIBMA polymer length on lipid nanodisc formation and membrane protein extraction.

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Detergent-free isolation of functional G protein-coupled receptors into nanometric lipid particles.

Biochemistry. 2016; 55: 38-48

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Detergent-free solubilisation & purification of a G protein coupled receptor using a polymethacrylate polymer.

Biochim. Biophys. Acta Biomembr. 2021; 1863183441

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Detergent alternatives: membrane protein purification using synthetic nanodisc polymers.

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Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins.

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Biophysical characterization of membrane proteins in nanodiscs.

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The charge properties of phospholipid nanodiscs.

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Rapid preparation of nanodiscs for biophysical studies.

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Express incorporation of membrane proteins from various human cell types into phospholipid bilayer nanodiscs.

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Direct solubilization of heterologously expressed membrane proteins by incorporation into nanoscale lipid bilayers.

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Co-incorporation of heterologously expressed Arabidopsis cytochrome P450 and P450 reductase into soluble nanoscale lipid bilayers.

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Nanodisc-solubilized membrane protein library reflects the membrane proteome.

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Cryo-EM structure of the native rhodopsin dimer in nanodiscs.

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Transducin activation by nanoscale lipid bilayers containing one and two rhodopsins.

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Emerging structural insights into GPCR–β-arrestin interaction and functional outcomes.

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Cryo-EM structure of an activated GPCR–G protein complex in lipid nanodiscs.

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Covalently circularized nanodiscs for studying membrane proteins and viral entry.

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Circularized and solubility-enhanced MSPs facilitate simple and high-yield production of stable nanodiscs for studies of membrane proteins in solution.

FEBS J. 2019; 286: 1734-1751

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Nanodiscs incorporating native β1 adrenergic receptor as a novel approach for the detection of pathological autoantibodies in patients with dilated cardiomyopathy.

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Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins.

Sci. Rep. 2020; 10: 1130

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A tetrameric assembly of saposin A: increasing structural diversity in lipid transfer proteins.

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Saposins: structure, function, distribution, and molecular genetics.

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Structure of saposin A lipoprotein discs.

PNAS. 2011; 109: 2908-2912

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An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.

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Purification of a native nicotinic receptor.

Methods Enzymol. 2021; 653: 189-206

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Footprinting mass spectrometry of membrane proteins: ferroportin reconstituted in saposin A picodiscs.

Anal. Chem. 2021; 93: 11370-11378

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Interactions of a bacterial RND transporter with a transmembrane small protein in a lipid environment.

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Fusion protein strategies for cryo-EM study of G protein-coupled receptors.

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Structure of the native muscle-type nicotinic receptor and inhibition by snake venom toxins.

Neuron. 2020; 106: 952-962

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Structure and gating mechanism of the α7 nicotinic acetylcholine receptor.

Cell. 2021; 184: 2121-2134

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Agonist selectivity and Ion permeation in the α3β4 ganglionic nicotinic receptor.

Neuron. 2019; 104: 501-511

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Membrane protein extraction and purification using partially-esterified SMA polymers.

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Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer.

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Detergent-free extraction of a functional low-expressing GPCR from a human cell line.

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Membrane protein extraction and purification using styrene-maleic acid (SMA) copolymer: effect of variations in polymer structure.

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Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K⁺ channel: the power of native nanodiscs.

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Purification of membrane proteins free from conventional detergents: SMA, new polymers, new opportunities and new insights.

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Structure and activity of lipid bilayer within a membrane-protein transporter.

PNAS. 2018; 115: 12985-12990

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Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure.

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Cryo-EM structures of Escherichia coli cytochrome bo₃ reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site.

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Nature. 2018; 557: 123-126

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An acid-compatible co-polymer for the solubilization of membranes and proteins into lipid bilayer-containing nanoparticles.

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Bioinspired, size-tunable self-assembly of polymer–lipid bilayer nanodiscs.

Angew. Chem. Int. Ed. 2017; 56: 11466-11470

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Formation of pH-resistant monodispersed polymer–lipid nanodiscs.

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Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

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Measurement of residual dipolar couplings using magnetically aligned and flipped nanodiscs.

Langmuir. 2022; 38: 244-252

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Magnetic alignment of polymer macro-nanodiscs enables residual-dipolar-coupling-based high-resolution structural studies by NMR spectroscopy.

Angew. Chem. Int. Ed. 2019; 58: 14925-14928

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Solubilization of membrane proteins into functional lipid-bilayer nanodiscs using a diisobutylene/maleic acid copolymer.

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Spontaneous lipid nanodisc fomation by amphiphilic polymethacrylate copolymers.

J. Am. Chem. Soc. 2017; 139: 18657-18663

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Synthesis, characterization, and nanodisc formation of non-ionic polymers.

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A comparison of SMA (styrene maleic acid) and DIBMA (di-isobutylene maleic acid) for membrane protein purification.

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Functional solubilization of the β2-adrenoceptor using diisobutylene maleic acid.

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Enhancing the stability and homogeneity of non-ionic polymer nanodiscs by tuning electrostatic interactions.

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Non-ionic inulin-based polymer nanodiscs enable functional reconstitution of a redox complex composed of oppositely charged CYP450 and CPR in a lipid bilayer membrane.

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A split-intein-based method for the efficient production of circularized nanodiscs for structural studies of membrane proteins.

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Optimization of the production of covalently circularized nanodiscs and their characterization in physiological conditions.

Langmuir. 2018; 34: 3525-3532

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One-step construction of circularized nanodiscs using SpyCatcher-SpyTag.

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